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Determination of astragaloside in Anduolin Capsule by HPLC-ELSD
Author(s): 
Pages: 383-385
Year: Issue:  4
Journal: Drugs & Clinic

Keyword:  安多霖胶囊黄芪甲苷高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法;
Abstract: 目的 建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定安多霖胶囊中黄芪甲苷的方法.方法 采用Ultimate色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-超纯水(32∶68),体积流量为1 mL/min,漂移管温度40℃,蒸发光散射检测器雾化气体为氮气,体积流量为2.7 L/min,载气压力350 kPa,进样量20 μL.结果 黄芪甲苷在1.59~10.60μg呈良好的线性关系,r=0.999 5,平均回收率为99.11%,RSD为2.58%(n=6).结论 本方法简便准确,重现性好,可用于安多霖胶囊的质量控制.
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