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Sequencing and Phylogenetic Analysis of the 16S rDNA of Ralstonia solanacearum MR111 in Mulberry
Pages: 29-30,39
Year: Issue:  17
Journal: Hunan Agricultural Sciences

Keyword:  青枯雷尔氏菌MR11116S rDNA系统进化分析;
Abstract: 对从发病的桑树中分离出的菌株MR111的16S rDNA进行PCR扩增和序列分析.结果表明:对菌株MR111进行16SrDNA的PCR扩增,获得了1 435 bp 16S rDNA序列.NCBI在线BLAST分析表明,该序列与茄科雷尔氏菌PC-3、RSGC-1等序列的同源性达95%.对28个茄科雷尔氏菌菌株的16S rDNA序列进行系统进化分析,MR111与来自空心菜(GY0501)、番茄(ZCz-3)等的菌株聚合在一个簇群,证实该菌株为青枯雷尔氏菌.命名为桑青枯雷尔氏菌MR111a.
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