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Construction of Full-length cDNA Library for Midgut and Hindgut of Coptotermes formosanus Shiraki by CAP-trapper Method
Pages: 6-9
Year: Issue:  17
Journal: Hunan Agricultural Sciences

Keyword:  台湾乳白蚁中后肠CAP-trapper法cDNA文库;
Abstract: 通过CAP-trapper法构建台湾乳白蚁的中后肠全长cDNA文库,经过中后肠总RNA的提取、mRNA的纯化与分离、cDNA双链的合成、cDNA大片段的分级分离与回收、载体的连接,体外包装等步骤,获得了滴度为1.6×106 pfu/mL,重组率为98.7%,库容量为5.6×107cfu的初级文库,扩增后文库滴度为8.5×109 pfu/mL,插入片段平均大小为2.1 kb,插入片段全长率约为44%.这表明利用该方法构建台湾乳白蚁中后肠cDNA文库切实可行.
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