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Flagellar identification and sequence analysis of flic gene of Pseudomonas aeruginosa isolated from mink
Author(s): 
Pages: 749-751
Year: Issue:  9
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  水貂铜绿假单胞菌鞭毛分型flic基因克隆序列分析;
Abstract: 为分析铜绿假单胞菌(PA)分离株鞭毛蛋白基因(flic)之间的差异,本研究通过PCR方法扩增由山东地区发病水貂的肺脏组织中分离的29株分离株flic基因的部分片段,根据其片段长度确定PA的鞭毛类型.选取两种类型代表株(PASD01、PASD03),采用PCR方法扩增flic全基因进行序列分析.结果表明,PASD01分离株与国外A型PA flic基因序列同源性为99.6%~99.7%,PASD03分离株与B型PAflic基因的序列同源性为98.9%~99.5%,而两个分离株之间的序列同源性为76%.本研究首次从水貂病变组织中克隆得到两种鞭毛蛋白类型的flic基因,该基因在各型鞭毛菌株中高度保守,为进一步研制鞭毛亚单位疫苗提供实验依据.
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