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Development of SYBR Green Ⅰ fluorescent quantitation assay for detection of Trypanosoma equiperdum
Pages: 724-727
Year: Issue:  9
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  马媾疫锥虫SYBR Green Ⅰ荧光定量;
Abstract: 为建立一种特异、快速的媾疫锥虫检测方法,本研究通过PCR方法从马媾疫锥虫动基体基因组中扩增得到395 bp的特异的保守序列,将其克隆到pMD-18T载体中构建重组质粒标准品,以10倍倍比稀释的质粒标准品为模板,进行SYBR Green Ⅰ荧光定量PCR扩增并制作标准曲线,建立马媾疫锥虫荧光定量PCR的检测方法.结果表明:该方法的检测灵敏度可达到1拷贝/μL,并且与马属动物其他传染病无交叉反应.其组间及组内变异系数分别小于3.183%和3.842%.该方法的建立为快速及特异性检测马媾疫锥虫提供了有效的方法.
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