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Differentiation of Umbilical Cord Mesenchymal Stem Cells (UCMSCs) Derived from Chicken into Isletβ-cells in vitro
Author(s): 
Pages: 123-129
Year: Issue:  4
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  脐带间充质干细胞分离培养鉴定诱导分化;
Abstract: 为了建立鸡脐带间充质干细胞(Umbilical cord mesenchymal stem cells,UCMSCs)体外培养体系并对其进行生物学特性研究.取11 ~15日龄鸡胚,脐带分离获得UCMSCs,在L-DMEM培养基中培养,观察其形态.通过传代、生长曲线和周期分析其增殖情况,并研究体外诱导分化潜能.结果表明,鸡UCMSCs可在体外扩增培养,目前传代至P30,表达CD29、CD44和CD71,并具有向胰岛β样细胞诱导分化的潜能.鸡脐带中可以分离出UCMSCs,能够在体外培养,并可诱导分化为胰岛β样细胞.
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