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Optimization ultrasonic extraction process of total saponins from Clematis tangutica(Maxim.) Korsh by response surface methodology
Author(s): NIU Jiang-jin 1, 2, WANG Ying 1, 2, ZHANG Lin 1, 2, SHAO Yun 1, MEI Li-juan 1, TAO Yan-duo 1, *
Pages: 199-
202,205
Year: 2012
Issue:
20
Journal: Science and Technology of Food Industry
Keyword: 甘青铁线莲; 总皂苷; 超声提取; 响应面法;
Abstract: 利用单因素实验及响应面法优化确定甘青铁线莲总皂苷的提取参数条件。通过单因素实验确定乙醇浓度、超声时间、料液比三因素的最佳水平。以甘青铁线莲总皂苷提取得率为响应值进行Box-Behnken中心组合实验设计,建立铁线莲总皂苷提取得率的二次回归方程,得到优化组合条件。响应面法分析结果表明,当乙醇体积分数为80%,超声提取时间50min,料液比1:30时,铁线莲总皂苷提取得率最大为15.78mg/g,接近于模型预测值16.19mg/g。
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