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Genetic Variation Analysis of Seven Geographic Populations of Oedaleus asiaticus B.Bienko from Inner Mongolia based on Mitochondrial 16S rRNA Gene Sequences
Author(s): 
Pages: 54-59
Year: Issue:  4
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  亚洲小车蝗地理种群mtDNA16S rRNA基因遗传变异;
Abstract: 为了解内蒙古草原优势害虫亚洲小车蝗( Oedaleus asiaticus)的种群状况,采用线粒体DNA (mtDNA) 16S rRNA基因测序技术对7个不同地理种群的亚洲小车蝗的种群结构和遗传变异进行研究.通过对亚洲小车蝗28个个体的线粒体16S rRNA基因进行测序,获得1个长度为289 bp的同源序列,通过编辑,剪切有267 bp的序列基可用于这28个个体的比较.在267 bp的序列中,A+T约占69.9%,A+T含量明显高于G+C含量.其中有20个变异位点,约占所测核苷酸总数的7.49%,密码子第3位点上的变异最多共检测出18个单倍型.以斑腿蝗科的鼓翅皱膝蝗Angaracris barabensis和槌角蝗科的宽须蚁蝗Myrmeleotettix Palpalis作外群构建NJ和UPGMA分子系统树.聚类结果表明,亚洲小车蝗mtDNA 16S rRNA基因序列不同个体间存在一定的分歧,呈现平行分布,没有明显的地理分布簇群,遗传变异与地理距离无明显相关性.
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