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Isolation and Express Analysis of FaCO-2, a CO Homologue from Strawberry
Author(s): 
Pages: 1-6
Year: Issue:  4
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  草莓CO同源基因分离表达;
Abstract: 以草莓(Fragaria×ananassa Duch.)品种花姬为试材,通过PCR和RT-PCR方法克隆出草莓CO同源基因FaCO-2的CDS和DNA全长序列,在此基础上通过实时定量PCR的方法检测了其在草莓植株各组织和花器官中的表达.结果表明,该基因DNA序列长度为1 882 bp,含1个内含子,其CDS长度为1 146 bp,编码382个氨基酸,与其他物种的CO同源基因氨基酸序列有较高的同源性,生物信息学分析表明该蛋白分子量为42 021.69 D,等电点pI=5.49.实时定量RT-PCR结果表明,在不同的组织、花器官中,FaCO-2的表达量存在差异,在充分展开的较大叶片中的表达量最高;在4轮花器官中,只在萼片中表达,而其他花器官中不表达.
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