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Study on determination of expansin in kiwi by HPLC
Author(s): MA Ji-Wei
Pages: 2340-
2341
Year: 2012
Issue:
10
Journal: Chinese Journal of Health Laboratory Technology
Keyword: 猕猴桃; 膨大素; HPLC;
Abstract: 目的:建立猕猴桃中膨大素的高效液相色谱测定法。方法:以甲醇-水(65∶35)为流动相,用HY-PERSIL C18(4.6 mm×150 mm,5μm)进行分离,于波长265 nm处检测。结果:线性范围为5μg/L~100μg/L(r=0.9999),回收率为98.5%,RSD为1.4%。结论:用该方法测定膨大素含量,结果稳定,重现性好,可操作性强,适于猕猴桃中膨大素含量的质量控制。
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