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Simultaneous Determination of Cyromazine and Melamine in Green Vegetables With HPLC
Author(s): 
Pages: 595-598
Year: Issue:  5
Journal: Rural Eco-Environment

Keyword:  环丙氨嗪 三聚氰胺 青菜 固相萃取 高效液相色谱;
Abstract: 研究并建立了同时测定青菜中环丙氨嗪和三聚氰胺残留量的高效液相色谱紫外检测分析方法。冷冻干燥并均质后的青菜样品经V(氨水)∶V(甲醇)=5∶95的混合溶液超声辅助浸提、离心、旋转蒸发、固相萃取等浓缩净化处理后,用高效液相色谱定量。分离色谱柱为Agilent NH2柱,流动相为V(乙腈)∶V(水)=90∶10的混合溶液,流速为1 mL·min-1,检测波长为214 nm。环丙氨嗪和三聚氰胺的标准曲线在0.05~10.0 mg·L-1范围内与其峰面积线性良好,相关系数不低于0.999 5,在0.2~4.0 mg·kg-1添加范围内,环丙氨嗪和三聚氰胺的平均回收率分别为81.26%~87.69%和78.24%~82.33%,相对标准偏差分别为2.75%~6.18%和3.99%~6.67%,方法的最低检测浓度分别为11.30和20.38μg·kg-1。该方法操作简单且灵敏度高,适用于青菜样品中环丙氨嗪和三聚氰胺残留检测。
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