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HPLC determination of kakkalide in Puerariae lobata Ohwio from different areas
Author(s): 
Pages: 435-437
Year: Issue:  6
Journal: Central South Pharmacy

Keyword:  高效液相色谱法葛花葛花苷;
Abstract: 目的 建立高效液相色谱法测定葛花中葛花苷含量方法.方法 采用依利特Hypersil ODS (4.6 mm×150 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸(17∶83),流速为1mL·min-1,柱温为35℃,检测波长;265 nm,进样量:10μL.结果 葛花苷在0.102 4~1.229 mg·mL-1内与峰面积线性关系良好,平均回收率为100.9%,RSD为1.7%.结论 所建立之方法准确可靠、灵敏度高、专属性强,可用于测定葛花中葛花苷的含量.
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