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Prokaryotic expression of Neisseria gonorrhoeae NspA and its antigenicity
Author(s): 
Pages: 395-398
Year: Issue:  5
Journal: Chinese Journal of Microbiology and Immunology

Keyword:  Neisseria gonorrhoeaeNspAAntigenicityFast detection technique;
Abstract: 目的 研究淋病奈瑟菌表面蛋白A(NspA)的抗原性及其在淋病快速诊断技术中的应用价值.方法 用PCR技术克隆淋病奈瑟菌NspA基因,构建其原核表达载体,在大肠杆菌中表达重组NspA,表达产物复性后免疫小鼠,Western blot、ELISA分别检测免疫血清与淋病奈瑟菌细胞裂解液中NspA及与淋病奈瑟菌全细胞的结合能力.结果 NspA基因可在大肠杆菌中表达,纯化并复性的重组NspA(rrNspA)可在小鼠体内刺激产生高效价特异性抗体,抗rrNspA抗体可与淋病奈瑟菌完整细胞及细胞裂解液中NspA特异性结合.结论 获得的rrNspA及抗rrNspA抗体在建立淋病奈瑟菌抗体或抗原快速检测方法中具有潜在的应用价值.
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