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Analysis of Genetic Diversity of Apis cerana in Guangdong Province Based on Mitochondrial Cytochrome b Gene Sequences
Author(s): 
Pages: 4-6
Year: Issue:  9
Journal: Journal of Bee

Keyword:  Guangdong provinceApis ceranamitochondrial DNAcytochrome bGenetic variation and Genetic differentiation;
Abstract: 采用PCR产物直接测序法对广东省3个地区的89群东方蜜蜂线粒体DNA细胞色素b基因(Cytb)序列进行了变异检测,并利用相关软件对所得数据进行群体遗传变异和遗传分化分析。所得序列长度为420bp,序列中的A,T,C,G平均含量分别为33.2%,44.0%,14.3%,8.5%;共检测到13个变异位点,其中转换变异位点12个,颠换变异位点1个,并且变异位点大多数发生在密码子第三位,无碱基插入或缺失;碱基组成A+T含量高,占77.2名,呈现明显的A+T偏倚性;3个地区种群间的遗传距离为0.0023、0.0020、0.0019;共定义14种单倍型,单倍型多样度为0.781±0.032,核苷酸多样度为0.004±0.0003。结果揭示广东省东方蜜蜂遗传多样性较为丰富,且存在一定的遗传分化。
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