Interaction between Actin and Cisplatin or Transplatin
Author(s): Yang Yong, Duan Lili, Li Zhifen, Zeng Huihui, Wang Baohuai, Zhang Youmin
Pages: 1073-1079
Year: 2000 Issue: 12
Journal: ACTA PHYSICO-CHIMICA SINICA
Keyword: Actin; Cisplatin; Transplatin; 1H- NMR;
Abstract: The interaction between actin and cisplatin or transplatin has been studied by using the 1H- NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F- actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G- actin at low concentration and inhibits it at high concentration,while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin / transplatin to G- actin are discussed.It is probable that cisplatin binds with Cys- 10 and Cys- 285,while transplatin does not bind with Cys- 10.
Pages: 1073-1079
Year: 2000 Issue: 12
Journal: ACTA PHYSICO-CHIMICA SINICA
Keyword: Actin; Cisplatin; Transplatin; 1H- NMR;
Abstract: The interaction between actin and cisplatin or transplatin has been studied by using the 1H- NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F- actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G- actin at low concentration and inhibits it at high concentration,while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin / transplatin to G- actin are discussed.It is probable that cisplatin binds with Cys- 10 and Cys- 285,while transplatin does not bind with Cys- 10.
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