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Polygalacturonase from Rhizoctonia cerealis,the Pathogen of Wheat Sharp Eyespot and Characterization of Its Physiological and Biochemistry Properties
Author(s): JI Zhaolin, GUO Ju, ZHANG Lina, CHEN Xijun, TONG Yunhui, XU Jingyou
Pages: 569-
573
Year: 2012
Issue:
3
Journal: Journal of Triticeae Crops
Keyword: Rhizoctonia cerealis; Polygalacturonase; Characteristics;
Abstract: 为了解小麦纹枯病菌产生的多聚半乳糖醛酸酶(Polygalacturonase,PG)的理化性质,先用丙酮法提取PG粗蛋白,再经DEAE-Sepharose Fast Flow离子交换柱、Phenyl-Sepharose 6Fast Flow疏水柱和Sephadex G-75凝胶柱纯化,得到一种具有较高活性的PG纯蛋白。该蛋白分子量为41.78kD;等电点为5.34;含有糖基,含糖量为9.10%;含...
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