Induction of Polyploid in the Pacific Abalone by Caffeine-Heat Shock Treatments
Author(s): MAO Lian-Ju, WANG Zi-Chen, LIU Xiang-quan, LI Ya-juan, GAO Yue-Mian
Pages: 959-965
Year: 2000 Issue: 11
Journal: ACTA GENETICA SINICA
Keyword: Haliotis discus hannai Ino; polyploid induce; Caffeine; heat shock;
Abstract: Polyploid of Pacific abalone, Haliotis discus hannai Ino, was induced by blocking Polar body I with caffeine-heat shock treatments. Fertilized eggs were treated with caffeine concentrations at: 2.5mmol／L, 5mmol／L and 10mmol／L; heat shock temperatures at 24℃, 26℃, 28℃, lasting for 10min to 30min, total 5 periods of time,beginning at 10min post-pertilization. Both fertilizing and incubating at 21℃ sea water.The results of studies showed that the effective procedure for inducing triploid appeared to be 5mmol／L and 10mmol／L Caffeine concentration, heat shock tempera- ture 26℃, duration 10min to 15min, all beginning at 10min after insemination. The percentage of triploid was 64.6%～77.8%, Comprehensive efficiency index of polyploid induction was 0.57～0.61. Under the condition of 5mmol／L caffeine conce- ntration and the temperature 26℃ for 30 min duration at 10min post-fertilization, 2.8%～3.6% tetraploid embryos could be produced.
Pages: 959-965
Year: 2000 Issue: 11
Journal: ACTA GENETICA SINICA
Keyword: Haliotis discus hannai Ino; polyploid induce; Caffeine; heat shock;
Abstract: Polyploid of Pacific abalone, Haliotis discus hannai Ino, was induced by blocking Polar body I with caffeine-heat shock treatments. Fertilized eggs were treated with caffeine concentrations at: 2.5mmol／L, 5mmol／L and 10mmol／L; heat shock temperatures at 24℃, 26℃, 28℃, lasting for 10min to 30min, total 5 periods of time,beginning at 10min post-pertilization. Both fertilizing and incubating at 21℃ sea water.The results of studies showed that the effective procedure for inducing triploid appeared to be 5mmol／L and 10mmol／L Caffeine concentration, heat shock tempera- ture 26℃, duration 10min to 15min, all beginning at 10min after insemination. The percentage of triploid was 64.6%～77.8%, Comprehensive efficiency index of polyploid induction was 0.57～0.61. Under the condition of 5mmol／L caffeine conce- ntration and the temperature 26℃ for 30 min duration at 10min post-fertilization, 2.8%～3.6% tetraploid embryos could be produced.
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