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Issue:
Effect of antibiotics on growth and differentiation of callus in maize
Author(s):
LI Hai-xia
,
SUN Jin-hua
,
GUO Zhi-min
,
HU Yan-min
Pages:
16
-
21
Year:
2012
Issue:
1
Journal:
Journal of Henan Agricultural University
Keyword:
玉米
;
愈伤组织
;
抗生素
;
组织生长
;
Abstract:
以继代培养的玉米胚性愈伤组织为材料,研究了不同质量浓度的卡那霉素(Kan)、遗传霉素(C418)和头孢霉素(Cef)对胚性愈伤组织继代生长和分化的影响.结果表明:(1)当质量浓度大于3mg·L-1时,Kan对愈伤组织的生长具有一定抑制作用,其抑制作用在不同继代次数和不同基因型间具有明显差异;Kan明显降低了愈伤组织的分化出苗率;(2)G418具有显著抑制愈伤组织生长的负向效应.当G418的质量浓度...
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