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Pathological changes in midgut tissues of larvae of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae), after feeding Vip3Aa protein
Author(s): ZHANG Yan, LIANG Ge-Mei*, ZHANG Li-Li, WEI Ji-Zhen
Pages: 869-
876
Year: 2012
Issue:
7
Journal: Acta Entomologica Sinica
Keyword: 棉铃虫; Vip3Aa; Cry1Ac; 中肠组织; 病理变化;
Abstract: 为了进一步明确Vip3Aa的作用机制,利用透射电镜观察了棉铃虫4龄幼虫取食含Vip3Aa蛋白饲料后中肠杯状细胞的病理变化,并比较了其病变与取食含Cry1Ac饲料后棉铃虫组织病变的差异。取食含Vip3Aa饲料后,棉铃虫幼虫的中肠杯状细胞逐渐发生病变,主要表现为:微绒毛肿胀、脱落;细胞核核膜界限不清晰,染色质分布不均匀;线粒体变形、数量减少,内脊不清晰;内质网杂乱不规则、数量减少。与取食Cry1Ac的棉铃虫相比,取食Vip3Aa的棉铃虫中肠杯状细胞发生病变较为缓慢,在取食12h后才发现明显病变,随着取食时间的增加病变越来越明显;而取食Cry1Ac的棉铃虫2h后中肠杯状细胞就出现明显病变。本研究可为Vip3Aa作为新毒素策略的重要蛋白在棉铃虫Helicoverpa armigera综合防治中更好地发挥作用提供理论依据。
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