Author(s): DENG Yu, LIN Tao, SUN Chun-qing, ZHANG Hong-biao, ZHANG Rong, LONG Jin-xue, HUANG Lü, WEN Xin-tian, CAO San-jie, TONG Guang-zhi, YUAN Shi-shan, ZHENG Hao
Pages: 31-35
Year: 2012 Issue:  4
Journal: Progress in Veterinary Medicine
Abstract: 表达去除SD0803 E2基因跨膜疏水区的sE2蛋白并制备其抗体,为猪源BVDV诊断奠定基础.RT-PCR扩增SD0803株sE2基因,构建重组原核表达载体pGEX-4T-1-sE2,转化BL21(DE3),IPTG诱导蛋白表达,以纯化的重组蛋白为抗原免疫家兔制备多克隆抗体.采用间接ELISA、Western blot、间接免疫荧光等方法鉴定获得的抗体.结果显示,抗体效价最高可达1∶256 000...