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Interleukin-1β induced vascular endothelial cells EA.hy926 apoptosis via N-glycosylation modification on β1- integrin protein
Author(s): 
Pages: 921-925
Year: Issue:  7
Journal: Chinese Pharmacological Bulletin

Keyword:  EA.hy926糖基化凋亡;
Abstract: 目的 探讨IL-1β对血管内皮细胞损伤机制.方法 以EA.hy926血管内皮细胞为研究对象,利用不同浓度IL-1β刺激24 h,采用Western blot方法检测IL-1β对β1整合素、Bcl-2、Bax、Caspase-3及N-乙酞氨基葡萄糖转移-V(GnT-V)表达的影响;通过Lectin blot方法显示IL-1β对GnT-V所修饰的所有糖蛋白量的影响;免疫共沉淀方法检测IL-1β对β1整...
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