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Isolation and Identification of a Pig Bovine Viral Diarrhea Virus
Author(s): 
Pages: 416-423
Year: Issue:  3
Journal: Acta Veterinaria Et Zootechnica Sinica

Abstract: 从BVDV阳性仔猪病料中分离病毒,为开展猪源BVDV病原学研究奠定基础.将处理后BVDV阳性仔猪组织样品接种MDBK细胞,分离到1株猪源BVDV,命名为SD0803株.通过细胞培养、直接免疫荧光、5’-UTR与Npro PCR扩增、电镜观察、TCID50测定及对其分子进化特征加以分析.结果表明,该毒株在MDBK细胞上盲传至13代未出现细胞病变.在直接免疫荧光试验中呈阳性荧光信号.PCR扩增分别获得...
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