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Influences of Heat Shock Pretreatment on DNA Double-strand Breaks Damaged by High Temperature of V79 Cells
Author(s): Li Ming, Zhang Baoguo
Pages: 6-
8
Year: 2012
Issue:
1
Journal: Cancer Research on Prevention and Treatment
Keyword: 热休克预处理; γ-H2AX; 激光共聚焦显微镜; DNA双链断裂;
Abstract: 目的 研究热休克预处理对高温致V79细胞DNA双链断裂的影响.方法 培养V79细胞,分为对照组和39℃、40℃、41℃、42℃热休克预处理组,预处理后,恢复6h,再以43℃与45℃进行高温损伤处理,4%多聚甲醛固定,抗γ-H2AX抗体标记,然后羊抗鼠FITC二抗孵育1h,细胞核内DNA双链断裂斑点用激光共聚焦显微镜进行观察.结果 在激光共聚焦显微镜下观察,经统计:对照组细胞核内的DNA双链断裂数量...
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