Construction of an inducible expression vector based on α-galactosidase gene as a selection marker for Saccharomyces cerevisiae and its application
Author(s): ZHANG Wei, GUO Qin, RUAN Hui, HE Guo-qing
Pages: 43-47
Year: 2012 Issue: 1
Journal: Journal of Zhejiang University(Agriculture & Life Sciences)
Keyword: α-半乳糖苷酶基因; 筛选标记; 酿酒酵母; β-1; 3-1; 4-葡聚糖酶;
Abstract: 以α-半乳糖苷酶基因为筛选标记,构建GAL1诱导型启动子介导的酿酒酵母表达载体YGM-α-gal质粒,将枯草芽孢杆菌的β-1,3-1,4-葡聚糖酶基因克隆到此载体中,构建质粒YGMPA-α-gal,转化宿主酵母后,实现β-1,3-1,4-葡聚糖酶在酿酒酵母中的分泌表达.结果表明:在2％半乳糖诱导下,摇瓶发酵24 h后分泌表达的β-葡聚糖酶活性达到411.9 U.mL-1,而在培养60 h后,发酵液...
Pages: 43-47
Year: 2012 Issue: 1
Journal: Journal of Zhejiang University(Agriculture & Life Sciences)
Keyword: α-半乳糖苷酶基因; 筛选标记; 酿酒酵母; β-1; 3-1; 4-葡聚糖酶;
Abstract: 以α-半乳糖苷酶基因为筛选标记,构建GAL1诱导型启动子介导的酿酒酵母表达载体YGM-α-gal质粒,将枯草芽孢杆菌的β-1,3-1,4-葡聚糖酶基因克隆到此载体中,构建质粒YGMPA-α-gal,转化宿主酵母后,实现β-1,3-1,4-葡聚糖酶在酿酒酵母中的分泌表达.结果表明:在2％半乳糖诱导下,摇瓶发酵24 h后分泌表达的β-葡聚糖酶活性达到411.9 U.mL-1,而在培养60 h后,发酵液...
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