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Determination of Available Lysine in Milk-Based Infant Formula Powders with 2,4,6-Trinitrobenzene Sulfonic Acid
Author(s): Li Jun-yan1, Qiu Kai2, 3, Zhong Qi-ding2, 3, Yu Xiao-jin1, Ding Fu-qiang1, Xiong Zheng-he2, 3, Zhang Ze-sheng1 1 2 3
Pages: 156-
160
Year: 2012
Issue:
1
Journal: Food and Fermentation Industries
Keyword: 婴幼儿配方奶粉; 三硝基苯磺酸(TNBS); 有效赖氨酸;
Abstract: 采用三硝基苯磺酸法建立了乳基婴幼儿配方奶粉中有效赖氨酸的测定方法,研究了乙醚用量、水解时间以及三硝基苯磺酸的孵化时间对水解液吸光度的影响。方法线性范围为0.02~0.12 mg/mL,相关系数r2=0.999 3,检出限为3.72μg/mL,定量限为10.23μg/mL,样品重复性相对标准偏差RSD为1.55%~5.57%,回收率为86%~115%。
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