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Structural and functional analysis for leader transcription regulating sequence and its flanking sequences in 5\' untranslated region of porcine reproductive and respiratory syndrome virus
Author(s): 
Pages: 1-6
Year: Issue:  11
Journal: Animal Husbandry & Veterinary Medicine

Abstract: 为解析猪繁殖与呼吸综合征病毒(PRRSV)5’非翻译区(5\'UTR)的高级结构与功能,以北美株PRRSV感染性克隆为平台,通过定点突变PCR技术将其5\'UTR中一特殊高级调控元件的一级序列进行突变,以改变其二级茎环结构,从而解析该结构的基因调控水平.将突变DNA克隆转染入BHK-21细胞后利用免疫荧光及RT-PCR试验来研究拯救后突变病毒的转录、翻译特性,及通过空斑形态学及病毒生长曲线分析突变病毒...
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