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Effect of hydrocamptothecin on the proliferation and apoptosis of human fetal lung fibroblasts cell line HFL1 in vitro
Author(s): 
Pages: 4364-4366
Year: Issue:  24
Journal: The Journal of Practical Medicine

Keyword:  细胞凋亡羟基喜树碱人胚肺成纤维细胞细胞增殖;
Abstract: 目的:探讨羟基喜树碱(HCPT)对体外培养的人胚肺成纤维细胞(HFL1)增殖与凋亡的影响.方法:常规培养HFLl细胞,以未加HCPT的HFL1为对照组,以不同浓度梯度(0.008-32 mg/L)HCPT处理的HFL1作为实验组,采用MTT法检测HCPT干预24、48、72h后HFL1的增殖情况:光学显微镜观察干预后细胞的形态学变化;流式细胞仪检测细胞凋亡率;透射电镜下观察细胞凋亡的改变.结果:H...
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