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Preparation and application of double plasmid standard containing PML/RARα(L) fusion gene and reference gene ABL
Author(s): 
Pages: 482-485
Year: Issue:  5
Journal: Experimental and Laboratory Medicine

Keyword:  内参基因PML/RARα(L型)实时定量PCR质粒;
Abstract: 目的 建立一个含PML/RARα(L型)与ABL双基因质粒标准品,用于PML/RARαL型)的定量检测.方法 从NB4细胞中提取总RNA并逆转录为cDNA,以此为模板,设计PML/RARα(L型)与ABL基因特异性引物进行PCR,PCR产物经琼脂糖电泳、胶回收、PCMV4载体克隆后转化DH5α工程菌,提取质粒进行酶切、测序鉴定,提取质粒用于制作荧光定量PCR标准曲线,同时对质粒的稳定性能进行评价....
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