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A New Enzymatic End Point Assay of MPT-NAG Using Metal Ions as Stabilizer
Author(s): 
Pages: 40-43
Year: Issue:  10
Journal: Guangdong Trace Elements Science

Keyword:  MPT-NAG MPT N-乙酰-β-D-氨基葡萄糖苷酶 光度分析法;
Abstract: 建立了快速、简便、灵敏地测定MPT-NAG含量的酶法终点法。以金属离子为增稳剂,N-乙酰-β-D-氨基葡萄糖苷酶(NAG)催化6-甲基-2-硫代吡啶-N-乙酰-β-D-氨基葡萄糖苷(MPT-NAG)水解,生成产物6-甲基-2巯基吡啶(MPT),MPT在340 nm处有吸收峰,通过测定340 nm处吸光度值计算MPT含量。结果表明,该法线性范围0~50 mmol/L,平均回收率为101.5%,批内变异系数(CV)和批间变异系数分别为0.010 5~0.026 4、0.013 2~0.031 6,与高效液相法比较具有良好的相关性,线性回归方程和相关系数分别为y=1.02 1 x+0.882 1,r=0.998 9,简便、灵敏、可靠,适合实际推广应用。
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