Author(s): HE Guang-zhi, TIAN Wei-yi, GAO Ying, WANG Ping, WANG Wen-jia, XI Jin, YU Qi, WANG Qian-yu, HUANG Gao
Pages: 77-81
Year: 2011 Issue:  3
Journal: Journal of Natural Science of Hunan Normal University
Abstract: 目的:构建抗蛔虫人源单链抗体库,从中筛选建抗蛔虫人源特异性单链抗体.方法:分离10个患蛔虫病人的淋巴细胞,提取总RNA反转录为cDNA,PCR扩增人抗体重链(VH)和轻链(VL)可变区基因,采用SOE-PCR法将VH和VL片段随机拼接成scFv片段,并克隆入噬菌粒载体pCANTAB5E中,构建噬菌体单链抗体库.结果:初级库库容量为1.8×106,在大肠杆菌TG1中重组后得到1.6×106的次级抗体...