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Separation of highly repetitive DNA for construction HR Cot library of Equus asinus
Author(s): ZHAO Ying-jie, L(U) Pin, ZHOU Xiang-shan, ZHANG Yuan-xing
Pages: 563-
567
Year: 2011
Issue:
5
Journal: Journal of Huazhong Agricultural University
Keyword: 高度重复序列; 分离; 纯化; 羟基磷灰石色谱; Cot文库; 家驴;
Abstract: 为研究家驴基因组重复序列,通过2次羟基磷灰石柱层析,分离纯化家驴Cot 0-Cot 0.1之间的基因组高度重复DNA序列,用于构建家驴基因组Cot文库,并向GenBank提交了18条重复序列,其中4条含有LINEs序列片段,4条含有SINEs序列片段,3条含有卫星序列片段,4条含有简单重复序列片段,1条含有LTR-逆转录转座子片段,2条未知功能的重复序列.
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