Author(s): ZHANG Li-li, XU Xin-dong, PAN Wei-qing
Pages: 257-260
Year: 2011 Issue:  5
Journal: International Journal of Medical Parasitic Diseases
Keyword:  Schistosoma japonicum; Sj-TSP2; Allele; Polymorphism;
Abstract: 目的 分析日本血吸虫Sj-TSP2序列多态性.方法 抽提单条日本血吸虫成虫的总RNA,逆转录合成cDNA,PCR扩增目的片段.将目的片段克隆到载体pBlue2KSM中转化大肠杆菌,挑取10个阳性克隆测序.将获得的Sj-TSP2序列导入DnaSP序列分析软件进行分析,计算等位基因数目,鉴定单核苷酸多态性位点(single nuclear polymorphism,SNP).同时将序列导入Mega 4...