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Reprogramming umbilical cord matrix cells into pluripotent stem cells by using Sox2, Klf4, Oct4, c-Myc genes
Author(s): 
Pages: 3825-3827
Year: Issue:  21
Journal: The Journal of Practical Medicine

Keyword:  多潜能干细胞细胞编程脐带基质间充质细胞胚胎干细胞;
Abstract: 目的:探讨利用Sox2、Klf4、Oct4、c-Myc基因将脐带基质间充质细胞(UMC)编程为多潜能干细胞(iPS)的可能性.方法:原代分离培养UMC细胞,包装生产逆转录病毒感染UMC细胞,将感染后细胞接种到饲养层细胞培养,镜下观察细胞形态学变化.对编程后细胞进行碱性磷酸酶(AP)染色、检测细胞基因表达量和体內分化畸胎瘤实验.结果:获得原代UMC细胞,细胞被编程后形态类似于胚胎干细胞,细胞AP染色...
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