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Isolation of regulation genes related with liver regeneration by representational difference analysis
Author(s): 
Pages: 277-280
Year: Issue:  4
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  肝再生表达性差异显示分析 (RDA)基因克隆;
Abstract: 利用表达性差异显示分析 (RDA)技术, 研究大鼠2/3肝部分切除后1 h肝组织中基因的选择性表达, 建立了大鼠2/3肝部分切除术后1 h再生肝组织选择性表达基因EST库.该库约含3×104个独立克隆, 其中95%以上的克隆含有插入片段, 长度约200~700 bp不等, 对随机挑出的52个克隆的序列分析表明其中大多数基因与肝再生调控相关(38/52).10株未报道序列经RNA杂交证实, 其中6株与肝再生相关.
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