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Id2 translocation from nucleus to cytoplasm accelerating differentiation of skeletal muscle cells by regulating the expression of apoptosis inducing factor
Pages: 761-765
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  骨骼肌凋亡诱导因子Id2迁移分化再生免疫荧光大鼠;
Abstract: 目的 探讨Id2在骨骼肌再生中的作用机制.方法 用绿色荧光蛋白(GFP)-Id2-C2表达载体转染C2C12成肌细胞,对转染组和非转染组进行H2O2处理和2%马血清处理,用RT-PCR法观察两组细胞Id2基因表达的差异;Western blotting观察两组细胞的成肌分化相关蛋白的表达情况;免疫荧光显微镜观察正常组、纤维损伤组以及去神经支配组大鼠的骨骼肌中Id2和凋亡诱导因子(AIF)蛋白的表达...
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