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Expression of LGR5 in hepatoma cells, HCC tissue and its significance
Author(s): 
Pages: 802-806
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  LGR5肝癌免疫组织化学免疫印迹法实时荧光RT-PCR;
Abstract: 目的 探讨LGR5在4个肝癌细胞系、癌旁组织及肝癌组织中的表达,及在肝癌中的作用.方法 免疫印迹法及实时定量RT-PCR检测正常细胞和4种肝癌细胞中LGR5的表达;采用免疫组织化学检测36例肝癌及癌旁组织中LGR5及β-catenin的表达;利用免疫细胞化学检测HepG2细胞中LGR5的表达.结果 免疫印迹法及实时定量RT-PCR证实,与正常肝脏细胞相比,LGR5除了在HepG2细胞中等表达外,在...
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