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Neurofilament immune fluorescence staining: a new method for observing the morphology of the motor endplate
Pages: 774-776
Year: Issue:  5
Journal: Journal of Peking University(Health Sciences)

Abstract: 目的:了解应用神经微丝蛋白免疫荧光染色法观察运动终板的效果.方法:取SPF级大鼠6只,麻醉状态下取材双侧比目鱼肌神经入肌点组织,以多聚甲醛固定,冰冻切片,行神经微丝蛋白免疫荧光染色,荧光显微镜和激光共聚焦显微镜下观察运动终板形态,并用激光共聚焦显微镜软件对运动终板形态进行三维重建.结果:染色切片在荧光显微镜和激光共聚焦显微镜下均可观察到运动终板爪形结构形态,激光共聚焦显微镜采集图像进行三维重建后,...
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