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Establishment of Tetracycline-induced gene expression system based on the lentiviral vector
Author(s): 
Pages: 756-760
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  四环素慢病毒基因调控基因工程基因克隆荧光显微术;
Abstract: 目的 构建四环素诱导基因表达的3代慢病毒系统,为慢病毒介导的基因治疗提供实验依据.方法 将四环素调控的转录激活因子(rtTA和M2rtTA)分别克隆入带有新霉素(neo)筛选标记的慢病毒载体中,得到pELNS-rtTA-IRES-Neo和pELNS-M2rtTA-IRES-Neo质粒,将四环素顺式应答作用元件(TETO和TREpitt)和绿色荧光蛋白(GFP)克隆入带有杀稻瘟素(blasticid...
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