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Decrease of serum protein concentration difference by solid-phase ligand libraries
Author(s): 
Pages: 770-774
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  血清蛋白质组学固相配体文库簇集蛋白高丰度蛋白低丰度蛋白高效液相色谱法;
Abstract: 目的 探讨固相配体文库技术富集血清低丰度蛋白及提高质谱鉴定低丰度蛋白敏感性.方法 3组混合血清标本采用蛋白富集珠(ProteoMiner)处理,一维或二维电泳图谱分析比较处理前后血清图谱中的差异条带或蛋白质斑点,并于处理后血清的二维电泳图谱中显著增加的蛋白质斑点进行高效液相色谱法电喷雾串联质谱鉴定.结果 一维及二维电泳图谱表明,固相配体文库技术能显示更多的蛋白条带或蛋白质斑点;显著富集的蛋白质点...
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