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Effect of calcitonin gene-related peptide on RUNX2 expression in primary rat osteoblasts
Author(s): 
Pages: 652-656
Year: Issue:  5
Journal: Journal of Peking University(Health Sciences)

Keyword:  降钙素基因相关肽成骨细胞转录因子RUNX2大鼠;
Abstract: 目的:探讨外源性降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对大鼠颅盖骨来源成骨细胞RUNX2因子表达水平的影响,进一步了解CGRP促进成骨的作用机制.方法:用酶消化法培养原代大鼠成骨细胞并鉴定;MTT法筛选促进成骨细胞增殖的有效浓度,将含有不同浓度CGRP的条件培养基加入大鼠成骨细胞培养体系中,药物作用48 h后,用半定量RT-PCR和Wester...
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