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Ad-hDCT (tumor vaccine) inhibiting the proliferation of intracranial B16 melanoma cells in C57BL/6 mice
Pages: 787-791
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  Ad-hDCT黑色素瘤细胞系B16脑肿瘤免疫组织化学流式细胞术C57BL/6小鼠;
Abstract: 目的 观察肿瘤疫苗Ad-hDCT预防接种对移植到C57BL/6小鼠脑实质内的黑色素瘤B16细胞增殖的抑制作用.方法 40只C57BL/6小鼠随机分为2组:Ad-BHG对照组(20只)和Ad-hDCT免疫接种组(20只).借助小鼠脑立体定位仪行B16细胞脑内注射;采用大体观察、流式细胞术、石蜡切片HE染色及冷冻切片CD8及F4/80抗体免疫组织化学染色等方法观察肿瘤细胞的增殖情况.结果 Ad-h...
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