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Smad3 gene promoting proliferation of GCs in rats
Author(s): 
Pages: 766-769
Year: Issue:  6
Journal: Acta Anatomica Sinica

Keyword:  Smad3siRNA颗粒细胞增殖免疫组织化学大鼠;
Abstract: 目的 探讨Smad3基因对大鼠卵巢颗粒细胞增殖功能的影响.方法 21d SD雌性大鼠,腹腔注射孕马血清20IU/只,48h后收集卵巢颗粒细胞进行原代培养,用Smad3 基因特异的siRNA基因沉默后,采用免疫细胞化学法检测颗粒细胞中Smad3蛋白表达的变化,以判定基因沉默效率,Smad3基因沉默后用免疫细胞化学法检测增殖细胞核抗原(PCNA)及Cyclin D2蛋白表达的变化.结果 Smad3...
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