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Primarily identification of pear variants and analysis of their genetic relationship by SRAP markers
Author(s): XU Ao-jing, ZHU Li-wu, JIA Bing, HENG Wei, YE Zhen-feng
Pages: 463-
468
Year: 2011
Issue:
3
Journal: Journal of Anhui Agricultural University
Abstract: 为了鉴定砂梨变异类型在DNA水平上是否发生遗传变异,以砂梨的两个变异单株及25个梨代表品种为试材,通过正交试验设计优化了SRAP扩增体系,并从88对SRAP引物中筛选出22对适宜的引物进行了PCR扩增,找出差异性片段,并应用系统聚类法,分析其与其它梨品种的亲缘关系.结果表明,砂梨适宜的SRAP体系为25μL反应体积中Mg2+ 2.4 mmol·L-1、dNTPs 0.1 mmol·L-1、Taq酶...
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