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The Influence of Green Fluorescent Protein (GFP) on Genomic Stability in Colorectal Cancer Cells
Pages: 342-346
Year: Issue:  3
Journal: Acta Laser Biology Sinica

Keyword:  绿色荧光蛋白结肠直肠癌细胞微核核芽;
Abstract: 为探究绿色荧光蛋白(GFP)对结肠直肠癌细胞遗传物质稳定性是否存在影响,选取3种常用的结肠直肠癌细胞系HCT116,SW480,DLD-1;采用脂质体转染法把GFP转入细胞,统计几种细胞系微核、核芽的比例;分别比较各种细胞系自发微核、核芽和GFP组的差异.结果发现,HCT116细胞中对照组与GFP组的微核细胞率分别为3.24%、2.76%,核芽细胞率为0.24%、0.26%;SW480细胞中对照组...
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