Author(s): CHEN Guo-fu, DING Ting-ting, ZHAO Jun, WANG Ming-li, YIN Zong-sheng
Pages: 173-177
Year: 2011 Issue:  3
Journal: Acta Academiae Medicinae Wannan
Abstract: 目的:探讨人重组α干扰素(rhIFN-α)及联合应用顺铂对骨肉瘤细胞(MG-63)凋亡的影响.方法:将rhIFN-α和顺铂分别和联合作用于MG-63细胞,光镜下观察细胞形态学改变,用MTT检测MG-63细胞增殖抑制情况,流式细胞仪法检测MG-63细胞凋亡情况,Hoechest 33258染色和DNAladder检测细胞凋亡.结果:10 000 IU/ml rhIFN-α对细胞增殖抑制情况较明显(3...