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Chemiluminescence Enzyme Immunoassay for the Determination of Zearalenone in Corn
Pages: 160-165
Year: Issue:  5
Journal: Food and Fermentation Industries

Keyword:  玉米赤霉烯酮(ZEN) 间接竞争化学发光酶免疫法(CLEIA) 检测;
Abstract: 应用抗玉米赤霉烯酮单克隆抗体,以辣根过氧化物酶催化鲁米诺-过氧化氢化学发光体系,建立了一种简便、快速、高特异性的间接竞争化学发光酶免疫法用于检测玉米中的玉米赤霉烯酮。所建立的方法分析灵敏度为0.09 ng/mL,批内变异系数小于9.1﹪,批间变异系数小于14.7﹪,样品的加标回收率为79.6﹪~97.6﹪,所建立的标准曲线相关系数为0.9884,检测线性范围为0.104~1.69 ng/mL,检测时间为60 min。
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