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A Simple and Convenient Method in the Process of Chitinase Purification
Author(s): 
Pages: 83-87
Year: Issue:  5
Journal: Food and Fermentation Industries

Keyword:  几丁质酶 胶体几丁质 亲和吸附 离心 纯化;
Abstract: 传统的纯化微生物产几丁质酶的方法步骤繁琐、不易控制、得率较低。经过实验探索得出一种较简单、快速、并可批量处理的几丁质酶纯化法。将含有几丁质酶的发酵上清液与胶体几丁质混合后离心,几丁质酶随胶体几丁质沉降而与其他成分分离,用蒸馏水洗涤沉淀,再用蒸馏水重悬,放入30℃恒温箱温育72 h,胶体几丁质被完全水解,从而得到纯几丁质酶。此纯化方法条件温和,可保证纯化后的酶保持其生物活性。SDS-PAGE检测及比色法对其纯度检测表明几丁质酶纯化回收率达到88.53%。
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