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The Influence of Membrane’s Type and the Pretreatment Methods to the Detection of Staphylococcal Enterotoxin A,B and C in Raw Milk by Lateral Flow Test Strips
Author(s): 
Pages: 157-159,165
Year: Issue:  5
Journal: Food and Fermentation Industries

Keyword:  葡萄球菌肠毒素A、B、C 原料奶 横向侧流免疫检测;
Abstract: 对用于原料奶中葡萄球菌A、B、C型肠毒素现场快速检测的免疫层析试纸条进行了初步研究。研究内容主要包括层析膜和原奶预处理方法的选择及优化,通过实验,最终选择了型号为HF09002的层析膜,采用了2倍稀释的样品预处理方法,研制所得的3种免疫层析试纸条分别检测原奶中SEA的灵敏度为10 ng/mL,SEB的灵敏度为4 ng/mL,SEC的灵敏度为8 ng/mL,与3 MTM TecraTM微孔板法检测结果相比无显著性差异。
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