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Study on Elimination of Drug-resistance Plasmid of E.coli
Author(s): LI Ji-zong, ZHOU Bi-jun, MA Zhe, PENG Quan-hai, GAO Ying, ZHANG Ren-jun, WANG Kai-gong, WEN Ming
Pages: 36-
40
Year: 2011
Issue:
4
Journal: Progress in Veterinary Medicine
Abstract: 为了解耐药质粒消除对大肠埃希菌耐药性及其他生理特性的影响,参考前期试验分离出的8株携带aac(6\')-Ib质粒耐药基因的大肠埃希菌的药敏试验.选取1株耐药性最强菌株用高温及SDS进行耐药质粒消除,PCR检测aac(6\')-Ib基因.结果显示,耐药质粒成功消除,耐药性消失,且细菌在不含抗生素条件下培养,耐药性消除状态可稳定遗传;但在含卡那霉素条件下连续传代,其耐药性可迅速恢复,第4代即可产生耐药性....
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