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Determination of the Content of Total Saponins in Lilium brownii F E Brown var viridulum Baker.by UV Spectrophotometry
Author(s): 
Pages: 55-58
Year: Issue:  5
Journal: Guangdong Trace Elements Science

Keyword:  龙牙百合 总皂苷 紫外可见分光光度法 含量测定;
Abstract: 为建立龙牙百合总皂苷的测定方法,并确定不同产地龙牙百合中皂苷的含量,以便控制龙牙百合药材的质量,采用香草醛-高氯酸显色,在535 nm处,利用紫外可见分光光度法测定了10个批次的龙牙百合中总皂苷的含量。结果表明,10个不同产地龙牙百合中皂苷的含量,分别是:8.39%、8.11%、8.24%、7.62%、7.38%、9.02%、8.83%、8.87%、9.21%、9.17%,RSD分别为:1.22%、0.87%、0.93%、1.32%、0.86%、1.37%、0.94%、1.39%、1.55%、1.61%。结果表明,该法准确、可靠、稳定性好,可作为龙牙百合皂苷定量分析的有效方法。
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